Review



mcf 10a genomic dna  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    New England Biolabs mcf 10a genomic dna
    Mcf 10a Genomic Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf+10a+genomic+dna/AsiSI/pm37352273-85-6-15
    Average 96 stars, based on 259 article reviews
    mcf 10a genomic dna - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5.
    Article Snippet: The LINE sequence was inserted into the pCMV3 vector using KpnI and XbaI enzymes (Thermo Fisher Scientific, MA, USA). .. ENPP5 3’UTR sequence was amplified with MCF-10A genomic DNA, digested with AsiSi and XhoI enzyme (NEB, MA, USA), and inserted into pEZX-GA02 vector (GeneCopoeia, MD, USA). .. Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA) for plasmid DNA and Lipofetamine imax (Thermo Fisher Scientific, MA, USA) for miR-126-5p mimic (Genepharama, Suzhou, China) were used for transfection following manufacturer’s instructions.

    Article Title: New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5
    Article Snippet: The LINE sequence was inserted into the pCMV3 vector using KpnI and XbaI enzymes (Thermo Fisher Scientific, MA, USA). .. ENPP5 3’UTR sequence was amplified with MCF-10A genomic DNA, digested with AsiSi and XhoI enzyme (NEB, MA, USA), and inserted into pEZX-GA02 vector (GeneCopoeia, MD, USA). .. Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA) for plasmid DNA and Lipofetamine imax (Thermo Fisher Scientific, MA, USA) for miR-126-5p mimic (Genepharama, Suzhou, China) were used for transfection following manufacturer’s instructions.

    Amplification:

    Article Title: New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5.
    Article Snippet: The LINE sequence was inserted into the pCMV3 vector using KpnI and XbaI enzymes (Thermo Fisher Scientific, MA, USA). .. ENPP5 3’UTR sequence was amplified with MCF-10A genomic DNA, digested with AsiSi and XhoI enzyme (NEB, MA, USA), and inserted into pEZX-GA02 vector (GeneCopoeia, MD, USA). .. Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA) for plasmid DNA and Lipofetamine imax (Thermo Fisher Scientific, MA, USA) for miR-126-5p mimic (Genepharama, Suzhou, China) were used for transfection following manufacturer’s instructions.

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).

    Article Title: MicroRNA-205 increases the sensitivity of docetaxel in breast cancer
    Article Snippet: .. Briefly, a ~600 bp fragment carrying pre-miR-205 was amplified from MCF-10A genomic DNA by the Phusion® High-Fidelity DNA Polymerase enzyme (New England Biolabs, Ipswich, MA, USA) using the following PCR primers: miR-205-5.1, 5′-GAATTCCTTATCTGGGTGGCTGTTTTG-3′ and miR-205-3.1, 5′-GGTACCGCGGTGCTTTTTCCAATCTGC-3′. ..

    Article Title: New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5
    Article Snippet: The LINE sequence was inserted into the pCMV3 vector using KpnI and XbaI enzymes (Thermo Fisher Scientific, MA, USA). .. ENPP5 3’UTR sequence was amplified with MCF-10A genomic DNA, digested with AsiSi and XhoI enzyme (NEB, MA, USA), and inserted into pEZX-GA02 vector (GeneCopoeia, MD, USA). .. Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA) for plasmid DNA and Lipofetamine imax (Thermo Fisher Scientific, MA, USA) for miR-126-5p mimic (Genepharama, Suzhou, China) were used for transfection following manufacturer’s instructions.

    Expressing:

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).

    Polymerase Chain Reaction:

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).

    Article Title: MicroRNA-205 increases the sensitivity of docetaxel in breast cancer
    Article Snippet: .. Briefly, a ~600 bp fragment carrying pre-miR-205 was amplified from MCF-10A genomic DNA by the Phusion® High-Fidelity DNA Polymerase enzyme (New England Biolabs, Ipswich, MA, USA) using the following PCR primers: miR-205-5.1, 5′-GAATTCCTTATCTGGGTGGCTGTTTTG-3′ and miR-205-3.1, 5′-GGTACCGCGGTGCTTTTTCCAATCTGC-3′. ..

    Clone Assay:

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).

    Modification:

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).

    Plasmid Preparation:

    Article Title: Suppression of cell growth and invasion by miR-205 in breast cancer
    Article Snippet: .. To generate miR-205 expression vector, a ~600 bp fragment carrying pre- miR-205 was amplified from MCF-10A genomic DNA by the high fidelity polymerase Phusion enzyme (New England Biolabs, Ipswich, MA) using PCR primers The amplified fragment was first cloned into pCR8 (Invitrogen) and then subcloned into a modified pCMV vector carrying hygromycin resistance gene. .. To construct miR-205 lentiviral expression vector, the same miR-205 insert was released from pCMV vector and then subcloned into pCDH-CMV-MCS-EF1-copGFP (System Biosciences, Mountain View, CA).



    Similar Products

    mcf 10a genomic dna  (New England Biolabs)
    96
    New England Biolabs mcf 10a genomic dna
    Mcf 10a Genomic Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf+10a+genomic+dna/AsiSI/pm37352273-85-6-15
    Average 96 stars, based on 1 article reviews
    mcf 10a genomic dna - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results